Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-1)
인간 p53 단백질을 인식하는 DO-1 클론의 단일클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 단백질 A/G 정제, 보존제 무첨가, -20°C 보관. 연구용으로만 사용.
- 카탈로그번호
- 7157-MSM8-P1ABX
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DO-1 |
| Immunogen | Recombinant human wild type p53 protein expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Monkey or Rat.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 upregulates transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerization domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is critical for p53 activation and stabilization. p53 functions to block proliferation of damaged cells and acts as an anticancer agent. Phosphorylation at Ser392 is associated with tumor formation. p53 levels are low in normal cells but elevated in transformed cell lines, contributing to malignancy. Mutations in the TP53 gene are common in various cancers and lead to loss of tumor suppressor activity. Germline mutations can also occur in cancer-prone families such as those with Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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