Thermo Fisher Scientific CD147 Monoclonal Antibody (8D12), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8D12

Conjugate

APC APC APC

View additional formats

• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10714990

Product Specific Information

Description: The monoclonal antibody 8D12 recognizes CD147 which is a member of the immunoglobulin superfamily. It is also called EMMPRIN and basigin. CD147 has been characterized as a tumor cell collagenase stimulating molecule and has also been shown to have a role in cell adhesion. CD147 can be found in association with integrins alpha3 beta1 and alpha6 beta1 by co-immpunopreciptations. Additionally it is thought to play a role in T cell activation and thymocyte cycling. CD147 is expressed broadly on hematopoietic and non-hematopoietic cells, especially in tumor cells.

Applications Reported: This 8D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 8D12 antibody has been pre-titrated and tested by flow cytometric analysis or normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms. CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is also a member of the immunoglobulin superfamily. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.