Thermo Fisher Scientific Alpha-Smooth Muscle Actin Polyclonal Antibody
Rabbit polyclonal antibody targeting human alpha-smooth muscle actin; validated for WB, IHC(P), and ICC/IF; reacts with multiple species; purified by antigen affinity chromatography; supplied as liquid at 1 mg/mL in PBS with glycerol and BSA; suitable ...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Horse, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to Human Alpha-smooth Muscle Actin (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% Glycerol, 0.2% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802720 |
Target Information
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved and play essential roles in cell motility, structure, and integrity.
Alpha, beta, and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins regulate cell motility.
Smooth muscle actin is an alpha actin found in skeletal muscle and smooth muscle vessel walls, gut wall, myometrium, and myoepithelial cells of breast and salivary glands.
Actin filaments interact with myosin to assist in muscle contraction, cell motility, and cytokinesis.
Defects in the smooth muscle actin gene can cause familial thoracic aortic aneurysm type 6.
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells and appears in stress fibers of fibroblastic cells during wound healing and fibrocontractive diseases.
Multiple alternatively spliced variants of smooth muscle actin have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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