Thermo Fisher Scientific PPP1R1A Recombinant Rabbit Monoclonal Antibody (SN0756)
PPP1R1A 단백질을 표적하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분이 없는 포뮬레이션 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN0756 |
| Immunogen | Synthetic peptide within Human PPP1R1A aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809592 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening individual clones to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Protein phosphatase inhibitor-1 (PPP1R1A, also known as IPP-1 or I-1) regulates the phosphorylation of other proteins and is phosphorylated by cyclic AMP-dependent protein kinase at Threonine 35.
It is also phosphorylated by Cdk1, Cdk5, and MAPK at Serine 67. In striatal tissues, glutamate-dependent regulation of NMDA-type channels influences phosphorylation at Ser 67.
The localization and expression of IPP-1 suggest distinct roles in specific brain regions and developmental stages, independent of PP-1 regulation.
PP-1 binds both phosphorylated and dephosphorylated IPP-1, and conversion to a Mn++-dependent state may be involved in its regulation by IPP-1. IPP-1 attenuates glycogen phosphorylase activity and contributes to hormonal control of glycogen metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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