Thermo Fisher Scientific PPP1R1A Recombinant Rabbit Monoclonal Antibody (SN0756)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:5000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN0756

Immunogen

Synthetic peptide within Human PPP1R1A aa 1-50 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PPP1R1A,uniProtId:Q13522-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:171,antigenImageFileName:MA5-32310_PPP1R1A_Q13522-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32310_PPP1R1A_Q13522-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809592

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Protein phosphatase inhibitor-1 (also designated inhibitor of protein phospha-tase 1, IPP-1 and I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase at Threonine 35. In addition, the proline-directed kinases Cdk1, Cdk5, and mitogen-activated protein kinase (MAPK) mediate in vitro phosphorylation of IPP-1 at the phylogenetically conserved position Serine 67. In striatal tissues, glutamate-dependent regulation of N-methyl-d-aspartic acid-type channels influences IPP-1 phosphorylation at Ser 67. The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1). PP-1 binds to both phosphorylated and dephosphorylated IPP-1. Conversion of PP-1 to a Mn++-dependent state appears to play a role in its regulation by IPP-1. IPP-1 attenuates the activity of glycogen phosphorylase and is thought to be important in the hormonal control of glycogen metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.