Thermo Fisher Scientific IBA1 Recombinant Rabbit Monoclonal Antibody (145)
Recombinant rabbit monoclonal antibody recognizing human and mouse IBA1/AIF1. Validated for WB, ELISA, and IP. High specificity and lot-to-lot consistency. Preservative-free formulation suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 145 |
| Immunogen | Recombinant Human IBA1/AIF1 Protein (Met1–Pro147) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784951 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
This antibody shows specificity for Human IBA1/AIF1.
Target Information
AIF1 is induced by cytokines and interferon and is involved in the negative regulation of vascular smooth muscle cell growth, contributing to the anti-inflammatory response to vessel wall trauma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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