Thermo Fisher Scientific CD31 Monoclonal Antibody (TLD-3A12)
CD31(PECAM-1) 단백질을 인식하는 마우스 단클론 항체로, 주로 내피세포, 혈소판, 백혈구에서 발현 분석에 사용. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 단백질 A로 정제된 액상 형태, 1 mg/mL 농도. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 8 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | 2 publications |
| Flow Cytometry (Flow) | 1:10–1:100 | 1 publication |
| ELISA | Assay-dependent | - |
| Affinity Purification (AP) | - | 1 publication |
| Miscellaneous (PubMed) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rhesus monkey, Rat |
| Published Species | Hamster, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLD-3A12 |
| Immunogen | Activated, Lewis rat-derived microglial cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928130 |
Additional Formats Available:
- Biotin (Cat. MA5-16950)
- FITC (Cat. MA5-16952)
- PE (Cat. MA5-16954)
- Custom conjugation available upon request
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Rat CD31 antibody, clone TLD-3A12, recognizes rat PECAM-1 (CD31), a 661 amino acid type I transmembrane protein expressed primarily on endothelial cells, platelets, and leukocytes.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T-cell and B-cell signaling via dual immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation, these motifs serve as docking sites for protein-tyrosine phosphatases.
CD31 is widely expressed within the vascular compartment, mainly at intercellular junctions. Its N-terminal Ig-like domain mediates homophilic binding, critical for cell-cell interactions.
CD31 plays key roles in:
- Integrin-mediated cell adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Regulation of immune receptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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