Thermo Fisher Scientific Rb1 (Tumor Suppressor Protein) Monoclonal Antibody (RB1, 1754)
Rb1 종양억제 단백질을 인식하는 단클론 항체로, 인간 및 마우스 시료에 반응합니다. 포르말린 고정 조직의 IHC(P) 분석에 적합하며, 단백질 A/G로 정제된 액상 형태입니다. 4°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RB1, 1754 |
| Immunogen | Recombinant human Rb1 protein (amino acids 1–928 of the target protein) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine residues but not on tyrosine. It forms complexes with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may regulate transcription, and loss of its function can lead to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of the breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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