Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (ARC0521)
EpCAM 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 모노클로날 항체로, WB, IHC, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (ARC0521)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:800 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0521 |
| Immunogen | Recombinant fusion protein containing amino acids 1–100 of human EpCAM (P16422) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848970 |
Product Specific Information
Immunogen sequence:
MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS VGAQNTVICS KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein expressed in epithelial tissues (~40 kDa). It functions as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is observed in many human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is expressed on baso-lateral surfaces of most simple epithelia and various carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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