Thermo Fisher Scientific PHD1 Recombinant Rabbit Monoclonal Antibody (ARC0230)
Thermo Fisher의 PHD1 재조합 토끼 단클론 항체(ARC0230)는 인간, 마우스, 랫트 시료에서 HIF PHD1 단백질 검출에 적합합니다. Western blot과 ELISA에 사용 가능하며, 고순도의 액상 항체로 안정적인 결과를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0230 |
| Immunogen | A synthetic peptide corresponding to amino acids 1–100 of human Prolyl hydroxylase PHD1 (EGLN2) (Q96KS0) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849296 |
Product Specific Information
Immunogen sequence:
MDSPCQPQPL SQALPQLPGS SSEPLEPEPG RARMGVESYL PCPLLPSYHC PGVPSEASAG SGTPRATATS TTASPLRDGF GGQDGGELRP LQSEGAAALV
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 (known as PHD1, PHD2, and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits.
Hypoxia-inducible factor (HIF) is a transcriptional regulator important in oxygen homeostasis.
The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
HIF PHD1, expressed widely with highest levels in testis, functions as a cellular oxygen sensor and is important in cell growth regulation.
It localizes to the nucleus or cytoplasm and is detected in hormone-responsive tissues such as mammary, ovarian, and prostate epithelium.
HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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