Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), PE-Cyanine7, eBioscience
STAT1 Tyr701 인산화 형태를 특이적으로 인식하는 단클론 항체로, 인간 세포 내 염색 및 유세포 분석에 적합합니다. PE-Cyanine7 형광체 결합으로 고감도 검출 가능하며, 광민감성 주의가 필요합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), PE-Cyanine7, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KIKSI0803 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815117 |
Additional Formats
- Unconjugated
- eFluor 450
- eFluor 660
- PE
- PE-eFluor 610
- Request custom conjugation available
Product Specific Information
This KIKSI0803 monoclonal antibody recognizes STAT1 phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to cytokine receptors associated with JAK kinases. Phosphorylation at Tyr701 by JAK1/JAK2 is essential for STAT1 dimerization, nuclear translocation, and DNA binding.
- Specificity Determination: ELISA, flow cytometry, and western blotting
- Reported Applications: Intracellular staining followed by flow cytometric analysis
- Tested Protocols: Tested by intracellular staining using Protocol C (Fixation/Methanol). Protocol A and B are not compatible.
- Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
- Light Sensitivity: Protect from light due to photo-induced oxidation sensitivity.
- Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57).
- Excitation/Emission: 488–561 nm / 775 nm (Blue, Green, Yellow-Green lasers).
Target Information
STAT1 (Signal Transducer and Activator of Transcription 1) is a transcription factor activated by interferons and growth factors. It regulates genes involved in cell growth, apoptosis, immune response, and lipid metabolism. Located on chromosome 2, STAT1 activation occurs via phosphorylation by JAK kinases, enabling nuclear translocation and gene transcription regulation.
STAT1 responds to ligands including IFN-α, IFN-β, IFN-γ, IL-10, IL-6, IL-11, LIF, CNTF, G-CSF, EGF, PDGF, and CSF-1. Two alternatively spliced transcript variants encode distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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