Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience

Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.125 µg)/test

Publications

• View 9 publications (see references section)

Product Specifications

Available Formats

• APC
• FITC
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

This SRBCZX monoclonal antibody recognizes STAT5 when phosphorylated on tyrosine 694. STAT proteins are activated by cytokine receptor-associated JAK kinases, leading to nuclear translocation and transcriptional regulation. Phosphorylation at Tyr694 is essential for dimer formation and DNA binding.

• Specificity Determination: ELISA and flow cytometry
• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-titrated and validated for intracellular staining of stimulated human peripheral blood cells (5 µL/test, 100 µL final volume, 10⁵–10⁸ cells/test)
• Recommended Protocol: Protocol C (Two-step: Fixation/Methanol)
  • Protocol A and B not suitable for this antibody
  • Refer to Staining Intracellular Antigens for Flow Cytometry Protocol in BestProtocols® online resource

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
Excitation/Emission: 488–561 nm / 775 nm; compatible lasers: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT5 alpha is a transcription factor activated by cytokines and growth factors. Upon phosphorylation, it forms dimers and translocates to the nucleus to regulate gene expression. It mediates responses to IL2, IL3, IL7, GM-CSF, erythropoietin, and growth hormones. STAT5 alpha activation is implicated in tumorigenesis and anti-apoptotic signaling. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues.

For Research Use Only. Not for use in diagnostic procedures.

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