Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience
Phospho-STAT5 (Tyr694) 단백질을 인식하는 SRBCZX 단일클론 항체로, 인간 및 마우스 시료에 적합. PE-Cyanine7 형광체 결합으로 유세포분석용에 최적화. 고순도 친화 크로마토그래피 정제, 광민감성 주의 및 4°C 암저장 권장.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience
Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular staining)
- Tested Dilution: 5 µL (0.125 µg)/test
Publications
- View 9 publications (see references section)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SRBCZX |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573533 |
Available Formats
- APC
- FITC
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
This SRBCZX monoclonal antibody recognizes STAT5 when phosphorylated on tyrosine 694. STAT proteins are activated by cytokine receptor-associated JAK kinases, leading to nuclear translocation and transcriptional regulation. Phosphorylation at Tyr694 is essential for dimer formation and DNA binding.
- Specificity Determination: ELISA and flow cytometry
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Pre-titrated and validated for intracellular staining of stimulated human peripheral blood cells (5 µL/test, 100 µL final volume, 10⁵–10⁸ cells/test)
- Recommended Protocol: Protocol C (Two-step: Fixation/Methanol)
- Protocol A and B not suitable for this antibody
- Refer to Staining Intracellular Antigens for Flow Cytometry Protocol in BestProtocols® online resource
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
Excitation/Emission: 488–561 nm / 775 nm; compatible lasers: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
STAT5 alpha is a transcription factor activated by cytokines and growth factors. Upon phosphorylation, it forms dimers and translocates to the nucleus to regulate gene expression. It mediates responses to IL2, IL3, IL7, GM-CSF, erythropoietin, and growth hormones. STAT5 alpha activation is implicated in tumorigenesis and anti-apoptotic signaling. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.