Thermo Fisher Scientific GM-CSF Monoclonal Antibody (DAVKAT), PE, eBioscience

Thermo Fisher Scientific GM-CSF Monoclonal Antibody (DAVKAT), PE, eBioscience™

Applications

• Flow Cytometry (Intracellular Staining)

Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 1 publication

Product Specifications

Product Specific Information

This DAVKAT monoclonal antibody reacts with human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF).
Human GM-CSF is a 127 amino acid, differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF-α, IL-1, IL-2, and IFN-γ.
GM-CSF is found associated with the extracellular matrix and in membrane-bound formats as well. It stimulates proliferation, activation, and differentiation of macrophages, granulocytes, and their progenitors.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and BestProtocols®: Protocol A (two-step protocol for cytoplasmic proteins).
Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes.
The active form exists as a homodimer in the extracellular space.
This gene is located in chromosome region 5q31, associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia.
Other genes in the cluster include interleukins 4, 5, and 13.
It is involved in promoting tissue inflammation, and elevated cytokine levels have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome.
Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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