Thermo Fisher Scientific ENO1 Polyclonal Antibody
Thermo Fisher Scientific의 ENO1 Polyclonal Antibody는 인간 및 생쥐 시료에 반응하는 rabbit IgG 항체로, Western blot, IHC, ICC, ELISA, IP에 적합합니다. 고순도 친화 크로마토그래피 정제 제품이며, 다양한 세포 및 조직에서 ENO1 단백질 검출에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–434 of human ENO1 (NP_0014191) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.83 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901144 |
Product Specific Information
Positive Samples: PC-3, Ramos, NCI-H460, HeLa, THP-1, Mouse kidney, Mouse liver
Immunogen Sequence:
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Target Information
Enolase (2-phosphoglycerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It consists of three subunits: alpha, beta, and gamma.
Non-neuronal enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin.
ENO1 functions in glycolysis and is involved in processes such as growth control, hypoxia tolerance, and allergic responses. It may act as a receptor and activator of plasminogen on cell surfaces of leukocytes and neurons, stimulating immunoglobulin production.
ENO1 is used as a diagnostic marker for various tumors and hypoxic brain injury, and as a marker for endometriosis. Antibodies against alpha-enolase are associated with cancer-associated retinopathy syndrome (CAR) and identified as autoantigens in Hashimoto encephalopathy (HE), linked to Hashimoto thyroiditis (HT).
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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