Thermo Fisher Scientific RFWD2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Positive Samples: HepG2, MCF7, Mouse brain
• Immunogen Sequence:
  YYDLRNTKQP IMVFKGHRKA VSYAKFVSGE EIVSASTDSQ LKLWNVGKPY CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV

Target Information

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins.
E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis.
Ubiquitinates p53 independently of MDM2 or RCHY1. Functions as the essential RING domain subunit of larger E3 complexes.
Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival.
Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif.
[Source: UniProt]

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.