Thermo Fisher Scientific RYBP Monoclonal Antibody (OTI2F6), TrueMAB
RYBP 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot에 최적화되어 있습니다. 인간 RYBP 전체 길이 재조합 단백질을 면역원으로 사용하였으며, carrier-free 형태로 제공되어 결합 실험에 적합합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific RYBP Monoclonal Antibody (OTI2F6), TrueMAB
Applications and Tested Dilution
- Western Blot (WB): 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F6 |
| Immunogen | Full length human recombinant protein of human RYBP produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RYBP is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, essential for maintaining transcriptional repression of genes such as Hox throughout development.
This complex mediates monoubiquitination of histone H2A at Lys-119, leading to heritable chromatin modifications and gene silencing.
RYBP also plays roles in:
- X chromosome inactivation in XX females
- Regulation of TP53 stability and transcriptional activity
- Ubiquitin-mediated degradation of proteins such as FANK1, influencing apoptosis
- Transcriptional repression of E4TF1 activity
- Potential suppression of tumor growth and metastasis in breast cancer via SRRM3 down-regulation
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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