Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Polyclonal Antibody
ERK1/2 단백질의 인산화(Thr202, Tyr204) 상태를 검출하는 Rabbit Polyclonal 항체. Western blot 및 IHC에 사용 가능. 다양한 종에서 반응하며 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:2,000 |
| Immunohistochemistry (IHC) | 1:300–1:2,000 |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in many species including rat, mouse, zebrafish, xenopus, and chicken.
- Specificity: Erk1 and Erk2.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
They are activated by various stimuli such as growth factors, cytokines, virus infection, and G protein-coupled receptor ligands.
Upon activation, Raf phosphorylates MEK1/2, which then phosphorylates ERK1/2. Activated ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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