Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Polyclonal Antibody
ERK1/2의 인산화 형태(Thr202, Tyr204)를 인식하는 sheep polyclonal antibody로, WB와 IHC에 사용 가능. 인간, 생쥐, 랫드 반응성. Lyophilized 형태로 제공되며, 장기 보관 시 -20°C 권장. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 10–50 µg/mL
- Publications: View 2 publications
Immunohistochemistry (IHC)
- Tested Dilution: 10–50 µg/mL
- Publications: View 2 publications
Miscellaneous PubMed (Misc)
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in many species including rat, mouse, zebra fish, xenopus, and chicken.
- Specificity: Erk1 and Erk2.
Target Information
ERK1 and ERK2 are widely expressed and involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Various stimuli such as growth factors, cytokines, virus infection, and transforming agents activate the ERK1/2 pathways.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors including c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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