Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 570, eBioscience
FOXP3 단백질 인식용 단클론 항체(FJK-16s)로, eFluor 570 형광 표지된 제품입니다. 마우스, 랫, 개, 돼지, 소, 고양이 등 다양한 종에 반응합니다. 면역조직화학(IHC), 면역형광(ICC/IF) 등 연구용으로 적합하며, Treg 세포 연구에 활용됩니다.
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Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 570, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 12 |
| Immunohistochemistry (IHC) | - | 72 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 1 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 7 |
| Flow Cytometry (Flow) | - | 2 |
| Immunoprecipitation (IP) | - | 2 |
| ChIP assay (ChIP) | - | 2 |
| In Situ Hybridization (ISH) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | eFluor™ 570 |
| Excitation/Emission Max | 556/569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11220274 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, Biotin
- Brilliant Violet 421, 605, 711, 786
- eFluor 450, 506, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The FJK-16s antibody reacts with Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2) from mouse, rat, dog, porcine, bovine, and cat. Cross-reactivity with other proteins has not been determined. Foxp3 is a 49–55 kDa transcriptional regulator associated with regulatory T cells (Treg). The antibody epitope maps to amino acids 75–125 of mouse Foxp3.
Applications include immunohistochemistry (frozen and paraffin-embedded tissues). Recommended usage:
- Frozen mouse spleen: ≤5 µg/mL
- Paraffin-embedded tissue: ≤10 µg/mL with antigen retrieval (high or low pH)
Not validated for flow cytometry. Optimal dilution should be determined by the user.
Filter Recommendation:
For eFluor® 570 conjugates, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Alexa Fluor® 555 or TRITC filters are also suitable.
Excitation: 555 nm
Emission: 570 nm
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis, expressed in CD4⁺CD25⁺ regulatory T cells. It serves as a key marker for Treg cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome in humans. FOXP3 expression is also regulated by p53 in response to DNA damage in certain cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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