Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 570, eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 마커 분석에 적합합니다. eFluor 570 형광 표지로 IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능합니다. 인간, 마우스, 랫트 등 다종 반응성을 보이며, 고순도 액상 제형으로 제공됩니다.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 570, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 69 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 3 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 42 publications |
| Flow Cytometry (Flow) | - | 4 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219874 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- Alexa Fluor Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395, 496, 563, 615, 661, 737, 805)
- Brilliant Violet (421, 480, 605, 650, 711, 786)
- eFluor 450, 506, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a key proliferation marker. The Ki-67 labeling index is widely used in tumor studies to estimate the proportion of proliferating cells and predict cancer outcomes.
The SolA15 antibody also cross-reacts with human, non-human primate, and canine Ki-67.
Applications Reported:
Immunohistochemistry (frozen and paraffin-embedded tissues) and Immunocytochemistry.
Applications Tested:
Validated in ICC using fixed and permeabilized C2C12 cells at ≤1 µg/mL. Optimal dilution should be determined experimentally.
Filter Recommendation:
Use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Compatible with standard Alexa Fluor® 555 or TRITC filters.
Excitation / Emission: 555 nm / 570 nm
Target Information
Ki-67 is a nuclear protein expressed throughout the active cell cycle phases (late G1, S, G2, M) and absent in G0. It associates with euchromatin and heterochromatin regions, showing cell cycle–dependent localization. Ki-67 serves as a reliable proliferation marker and correlates strongly with tumor grade and growth rate. Commonly used in oncology and neuroscience for assessing cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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