Thermo Fisher Scientific ApoA1 Recombinant Rabbit Monoclonal Antibody (19H20L19)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Predicted to react with mouse and non-human primate based on sequence homology.
• Intact IgG appears on a non-reducing gel as ~150 kDa band; upon reduction generates ~25 kDa light chain and ~50 kDa heavy chain bands.
• For sandwich ELISA, can be paired with mouse monoclonal detection antibodies (MA5-14668, MA5-14670, MA5-14732) to detect recombinant human ApoA1 but not mouse ApoA1.
• Recommended to use biotinylated detection antibody followed by Streptavidin-HRP for increased sensitivity.
• Recombinant rabbit monoclonal antibodies are produced via in vitro expression systems using cloned antibody DNA sequences from immunoreactive rabbits.
• Advantages include enhanced specificity and sensitivity, lot-to-lot consistency, animal origin-free formulation, and broader immunoreactivity.

Target Information

Apolipoprotein A-1 (ApoA1) is a major protein component of high-density lipoprotein (HDL) in human plasma with a molecular mass of 24 kDa. It acts as a cofactor for enzymes involved in cholesterol metabolism and is primarily produced in the liver. Typical plasma concentration ranges from 270–380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.