
Thermo Fisher Scientific ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4)
Recombinant rabbit monoclonal antibody against ApoA2 for human and rat samples. Suitable for WB and ICC/IF applications. High specificity, lot-to-lot consistency, and animal origin-free formulation. Supplied as liquid, 0.5 mg/mL, stored at 4°C or -20°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 43H22L4 |
| Immunogen | Peptide corresponding to amino acids 60–71 of human ApoA2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532435 |
Product Specific Information
This antibody is predicted to react with non-human primate and mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems utilize antibody DNA sequences cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Apolipoproteins act as stabilizers of intact lipoprotein particles. At least nine polymorphic forms are known. Quantitative measurements of HDL, LDL, and VLDL particles in human serum help estimate coronary heart disease risk. Immunological measurements of apolipoproteins A-1 and B are considered more accurate indicators than lipoprotein particle measurements.
Apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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