Thermo Fisher Scientific E2F1 Monoclonal Antibody (8G9)
E2F1 단백질을 검출하는 Mouse IgG1 단일클론 항체로, WB, IHC, Flow Cytometry, ELISA에 적합합니다. Human 및 Mouse 시료에 반응하며, 농도 1 mg/mL의 액상 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G9 |
| Immunogen | Purified recombinant fragment of human E2F1 (amino acids 69–223) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538541 |
Product Specific Information
MA5-17070 targets E2F1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.
The immunogen is a purified recombinant fragment of human E2F1 (amino acids 69–223) expressed in E. coli.
MA5-17070 detects E2F1, which has a predicted molecular weight of approximately 46.9 kDa.
Target Information
The E2F1 protein is a member of the E2F family of transcription factors, which play a crucial role in cell cycle control and the action of tumor suppressor proteins.
E2F proteins contain conserved domains including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within transactivation domain)
E2F1, along with E2F2 and E2F3, possesses an additional cyclin-binding domain.
It binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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