Thermo Fisher Scientific E2F1 Monoclonal Antibody (8G9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8G9

Immunogen

Purified recombinant fragment of human E2F1 (amino acids: 69-223) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E2F1,uniProtId:Q01094-1,ncbiNodeId:9606,antigenRange:69-223,antigenLength:437,antigenImageFileName:MA5-17070_E2F1_Q01094-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17070_E2F1_Q01094-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538541

Product Specific Information

MA5-17070 targets E2F1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17070 immunogen is purified recombinant fragment of human E2F1 (amino acids: 69-223) expressed in E. Coli.

MA5-17070 detects E2F1 which has a predicted molecular weight of approximately 46.9kDa.

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.