Thermo Fisher Scientific QKI Monoclonal Antibody (N147/6), FITC
Thermo Fisher Scientific의 QKI Monoclonal Antibody (N147/6), FITC는 인간, 마우스, 랫트 QKI 단백질 검출용 항체로서 WB, IHC, ICC/IF에 사용 가능. FITC 형광 표지로 498/517 nm에서 발광하며 단백질 G로 정제된 고순도 제품. 연구용으로만 사용.
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Thermo Fisher Scientific QKI Monoclonal Antibody (N147/6), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N147/6 |
| Immunogen | Fusion protein amino acids 1–341 (full-length) of human QKI-5 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS (pH 7.4) with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932144 |
Additional Formats
- Unconjugated (MA5-27651)
- APC (MA5-45689)
- PE (MA5-45692)
- PerCP (MA5-45691)
- Request custom conjugation (Custom Form)
Product Specific Information
Mouse: 100% identity (341/341 amino acids identical).
Rat: 99% identity (339/341 amino acids identical).
90% identity with QKI-6, QKI-7, and QKI-7b.
1 µg/mL of MA5-45690 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 36–38 kDa.
This antibody was formerly sold as clone S147-6.
Target Information
This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles, and at low levels in the pancreas. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Sacsin protects against mutant ataxin-1. A pseudogene associated with this gene is located on chromosome 11. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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