Thermo Fisher Scientific QKI Monoclonal Antibody (N147/6), PerCP
QKI 단백질을 검출하기 위한 PerCP 결합 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 사람, 생쥐, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도. 형광 특성 482/675 nm, 보존제 없음, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N147/6 |
| Immunogen | Fusion protein amino acids 1–341 (full-length) of human QKI-5 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932145 |
Available Formats
Product Specific Information
- Mouse: 100% identity (341/341 amino acids identical)
- Rat: 99% identity (339/341 amino acids identical)
90% identity with QKI-6, QKI-7, and QKI-7b
- 1 µg/mL of MA5-45691 detects Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody
- Detects approximately 36–38 kDa
- Formerly sold as clone S147-6
Target Information
This gene encodes the sacsin protein, which contains UbL, DnaJ, and HEPN domains. It is highly expressed in the central nervous system, also found in skin, skeletal muscles, and at low levels in the pancreas. Mutations cause autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. Sacsin protects against mutant ataxin-1. A pseudogene is located on chromosome 11. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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