Thermo Fisher Scientific EphB1 Monoclonal Antibody (5F10A4)
Human 대상 EphB1 단백질 검출용 Mouse monoclonal antibody. Western blot, IHC, ELISA, IP에 사용 가능. Recombinant EphB1 fragment(aa19-133)로부터 제작. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| ELISA | 1:10,000 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F10A4 |
| Immunogen | Purified recombinant fragment of EphB1 (aa19–133) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10986585 |
Product Specific Information
MA5-15385 targets EphB1 in indirect ELISA, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of EphB1 (aa19–133) expressed in E. coli.
MA5-15385 detects EphB1, which has a predicted molecular weight of approximately 110 kDa.
Target Information
EPHB1 is a receptor tyrosine kinase belonging to the ephrin receptor family. Eph family kinases are involved in biological processes such as nervous system development, angiogenesis, and synapse formation.
EphB1 and related family members are type I transmembrane proteins with immunoglobulin, fibronectin type III, and cysteine-rich domains, as well as a cytoplasmic tyrosine kinase domain upstream of a SAM domain.
EphB family proteins bind ephrin-B class ligands, which are also transmembrane proteins capable of bidirectional signaling.
EphB1 is predominantly expressed in developing neural structures and vascular epithelium. Upon binding ephrin-B1, it regulates cell attachment and assembly.
Members of this family are implicated in neuronal and vascular cell targeting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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