Thermo Fisher Scientific RFC2 Monoclonal Antibody (OTI2B2), TrueMAB
RFC2 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, Flow Cytometry에 적합합니다. Human 종에 반응하며, Lyophilized 형태로 제공됩니다. 고순도 친화 크로마토그래피 정제, 안정적인 저장용 PBS 버퍼 포함.
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Thermo Fisher Scientific RFC2 Monoclonal Antibody (OTI2B2), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–234 of human RFC2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RFC2 encodes a member of the activator 1 small subunits family. DNA elongation by polymerase δ and ε requires accessory proteins PCNA and RFC.
Replication factor C (activator 1) is a protein complex of five distinct subunits. The RFC2 gene encodes the 40 kDa subunit responsible for ATP binding and may promote cell survival.
Disruption of RFC2 is associated with Williams syndrome. Alternatively spliced variants and a pseudogene on chromosome 2 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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