Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience

Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• APC
• APC-eFluor 780
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This NGZB monoclonal antibody reacts with mouse Granzyme B, a member of the granzyme serine protease family found in the granules of cytotoxic T cells and NK cells. Granzyme B is also known as CGL1 (cathepsin G-like-1) and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1). It plays a crucial role in inducing apoptosis of target cells via interaction with cytotoxic T cells through the mannose 6-phosphate receptor.

This antibody does not cross-react with human Granzyme B and is not blocked by GB11, indicating recognition of a distinct epitope.

Applications Reported: Intracellular staining and flow cytometric analysis.
Applications Tested: Validated using mouse splenocytes with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended concentration ≤ 0.125 µg/test (100 µL final volume). Optimal titration recommended.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Granzyme B is a serine protease found in cytotoxic T cells and NK cells. It induces apoptosis in target cells by cleaving caspase-3 and initiating the caspase cascade or through mitochondrial pathways by cleaving Bid protein. Granzymes (A–G in mouse) are stored in lytic granules of CTLs and NK cells and play key roles in immune-mediated cytotoxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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