Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience
마우스 Granzyme B를 인식하는 NGZB 단클론 항체로, FITC 형광 표지되어 세포 내 염색 및 유세포 분석에 적합. 인간 Granzyme B와 교차 반응하지 않으며, 청색 레이저(488 nm) 기반 형광 검출에 최적화. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 56 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NGZB |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10732989 |
Available Conjugate Formats
- APC
- APC-eFluor 780
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This NGZB monoclonal antibody reacts with mouse Granzyme B, a member of the granzyme serine protease family found in the granules of cytotoxic T cells and NK cells. Granzyme B is also known as CGL1 (cathepsin G-like-1) and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1). It plays a crucial role in inducing apoptosis of target cells via interaction with cytotoxic T cells through the mannose 6-phosphate receptor.
This antibody does not cross-react with human Granzyme B and is not blocked by GB11, indicating recognition of a distinct epitope.
Applications Reported: Intracellular staining and flow cytometric analysis.
Applications Tested: Validated using mouse splenocytes with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended concentration ≤ 0.125 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Granzyme B is a serine protease found in cytotoxic T cells and NK cells. It induces apoptosis in target cells by cleaving caspase-3 and initiating the caspase cascade or through mitochondrial pathways by cleaving Bid protein. Granzymes (A–G in mouse) are stored in lytic granules of CTLs and NK cells and play key roles in immune-mediated cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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