Thermo Fisher Scientific p14ARF Polyclonal Antibody
인간 CDKN2A 단백질의 100-173번 잔기 영역을 기반으로 제작된 p14ARF 폴리클로날 항체. Western blot에 적합하며 BT549 또는 HeLa 세포 추출물을 양성 대조군으로 권장. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 암 연구 및 세포주기 조절 연구에 활용 가능.
- 카탈로그번호
- PA146021
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to a region within residues 100–173 of the human CDKN2A protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_923425 |
Product Specific Information
By Western blot, PA1-46021 is specific for the ~16 kDa CDKN2A protein (CDKN2A tends to run slightly higher than the theoretical MW of 14 kDa).
Additional non-specific bands may be seen at ~25 and 47 kDa.
Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse).
These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression.
The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53.
The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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