Thermo Fisher Scientific CIC Polyclonal Antibody
CIC 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼와 0.02% sodium azide 함유. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,500 | View 5 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50 | View 1 publication |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate | – |
| ChIP assay (ChIP) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues 1500–1608 of the human Capicua (CIC) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2291736 |
Product Specific Information
- By Western blot, PA1-46018 is specific for the ~250 kDa CIC protein.
- CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa), which may explain the observed 250 kDa complex band.
- Suggested positive control: HeLa whole cell extract.
Target Information
This gene encodes a core component of the exosome complex. The mammalian exosome is required for rapid degradation of AU-rich element-containing RNAs but not for poly(A) shortening.
The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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