Thermo Fisher Scientific EIF4E Monoclonal Antibody (OTI6C11), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve an antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

eIF4E participates in several cellular processes such as enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export.
All eukaryotic mRNAs possess a 7-methylguanosine cap structure (m7GpppX) at their 5′ ends.
EIF4E functions as a eukaryotic translation initiation factor that directs ribosomes to this cap structure.
It is a 24-kDa polypeptide existing both freely and as part of the EIF4F complex.
EIF4E serves as the rate-limiting component in the eukaryotic translation apparatus and mediates mRNA–ribosome binding.
Other EIF4F subunits include EIF4A (50 kDa, with ATPase and RNA helicase activity) and EIF4G (220 kDa).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.