Thermo Fisher Scientific PDXK Monoclonal Antibody (OTI5H5), TrueMAB
PDXK 단백질을 표적으로 하는 Mouse IgG2a 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 시료 반응성이 검증되어 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5H5 |
| Immunogen | Full length human recombinant protein of human PDXK produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in amino acid and neurotransmitter metabolism.
The PDXK gene encodes a 312-amino acid polypeptide exhibiting pyridoxal kinase activity. A 1.5-kb PDXK transcript is expressed in all tested tissues and shows circadian oscillations.
In mice, Pdxk expression in liver and brain is regulated by the transcription factors Dbp, Hlf, and Tef, which also display circadian expression. Mice lacking these factors exhibit reduced brain PLP, serotonin, and dopamine levels, leading to increased susceptibility to epileptic seizures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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