Thermo Fisher Scientific POT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Gel Shift (GS)

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 450-500 of human POT1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2166764

Product Specific Information

Suggested positive control: HL-60, antigen standard for POT1 (transient overexpression lysate).

Target Information

POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5-TTAGGG-3 repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5-TAGGGTTAG-3 sequence. Its activity is TERT dependent but it does not increase TERT activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.