Thermo Fisher Scientific SARS Coronavirus Spike Protein Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

-

In vitro Assay (IV)

Assay-Dependent

-

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to resikdues 288-303 of the putative SARS Spike glycoprotein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.2% gelatin

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_1087217

Product Specific Information

The immunogen is 69%(11/16) homologous to SARS-COV-2 (COVID-19) Spike protein. The cross reactivity is not empirically tested.

Suggested positive control: transfected mouse melanoma cell lysate.

Target Information

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.