Thermo Fisher Scientific Desmin Polyclonal Antibody
Desmin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(P)에서 검증됨. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 0.5 mg/mL 농도의 액상 형태로 제공되며, 근육세포 내 Desmin 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the N-terminus surrounding Threonine (Thr76/Thr77) without phosphorylation of human Desmin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Cardiac Myocyte
- Cellular location: Cytoplasmic and membrane
Target Information
Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, being predominantly expressed in all types of muscle cells (cardiac, skeletal, and smooth muscle). Its expression is regulated in a stage- and tissue-specific manner, induced during terminal skeletal muscle cell differentiation.
In skeletal and cardiac muscle cells, Desmin is localized in the Z-disk region and at the intercalated disk. In smooth muscle, expression is more heterogeneous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, such as rhabdomyosarcomas and leiomyosarcomas. Additionally, during myocardial dysfunction, dramatic changes in Desmin distribution have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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