Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. Human에 반응하며, Mouse·Rat·Dog에 예측 반응성을 보입니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 556–939 of Human ZO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.98 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546345 |
Product Specific Information
- Recommended positive controls: HeLa, HCT116, HaCaT
- Predicted reactivity: Mouse (99%), Rat (99%), Dog (100%)
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins can also associate with claudin, occludin, and F-actin at tight junction strands, providing a linkage between the actin cytoskeleton and the tight junction.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, while ZO-1 is also expressed at cell junctions of cardiac myocytes.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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