Thermo Fisher Scientific RAD50 Polyclonal Antibody
RAD50 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ELISA에 적합합니다. 인간 및 생쥐 시료에 반응하며, DNA 이중가닥 절단 복구 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제 및 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–270 of human Rad50 (NP_0057232) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.56 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804098 |
Product Specific Information
- Positive Samples: NIH3T3, MCF-7, A-549, HT-29, Mouse lung
- Cellular Location: Chromosome, Nucleus, Telomere
Immunogen sequence:
MSRIEKMSIL GVRSFGIEDK DKQIITFFSP LTILVGPNGA GKTTIIECLK YICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GELIAVQRSM VCTQKSKKTE FKTLEGVITR TKHGEKVSLS SKCAEIDREM ISSLGVSKAV LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALE TLRQVRQTQG QKVKEYQMEL KYLKQYKEKA CEIRDQITSK EAQLTSSKEI VKSYENELDP LKNRLKEIEH NLSKIMKLDN
Target Information
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double-strand break repair.
Rad50 associates with Mre11 and p95 (Nibrin) to form a multiprotein complex essential for the repair process.
Upon DNA damage, Rad50 and Mre11 redistribute and co-localize within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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