Thermo Fisher Scientific A-Raf Polyclonal Antibody
Rabbit polyclonal antibody against human A-Raf protein. Validated for Western blot (1:500–1:1,000). Recognizes amino acids 371–606 of human ARAF. Supplied as liquid, 1.92 mg/mL, stored at -20°C. For research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Positive Samples: HeLa, Jurkat, HepG2
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 371–606 of human ARAF (NP_001645.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.92 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804097 |
Product Specific Information
Immunogen sequence:
GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGCRDQIIF MVGRGYLSPD LSKISSNCPK AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS AARLVP
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
A-Raf, a second member of the Raf gene family, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney, and at lowest levels in brain tissue.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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