Thermo Fisher Scientific A-Raf Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000
• Positive Samples: HeLa, Jurkat, HepG2

Product Specifications

Product Specific Information

Immunogen sequence:
GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGCRDQIIF MVGRGYLSPD LSKISSNCPK AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS AARLVP

Target Information

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
A-Raf, a second member of the Raf gene family, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney, and at lowest levels in brain tissue.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.