Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Super Bright 702, eBioscience
인간 CD20 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Super Bright 702 형광체로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 702 nm에서 방출됩니다. B세포 분석 및 면역표현 연구용으로 적합합니다.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Super Bright 702, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 6 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717137 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711
- eFluor 450 / 506
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 590 / 660
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation available
Product Specific Information
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells but not plasma cells. Low-level expression may occur on a subset of mature T cells. CD20 plays a role in B-cell activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended at 5 µL (0.5 µg) per test (100 µL final volume).
- Excitation / Emission: 405 nm / 702 nm (Violet Laser)
- Recommended Filter: 710/50 bandpass
Super Bright 702 is a tandem dye excited by the violet laser and emits at 702 nm. Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright conjugates to minimize non-specific polymer interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness and FRET efficiency.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding, making it a valuable target for therapeutic B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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