Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (P84), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (P84), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 2 publications

Product Specifications

Available Formats

• APC
• Biotin
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Custom conjugation available upon request

Product Specific Information

This P84 monoclonal antibody reacts with mouse CD172a (SIRPα), a cell surface glycoprotein with three Ig-like extracellular domains and two cytoplasmic ITIM motifs. These motifs recruit SHP-1 and SHP-2 phosphatases. CD172a is expressed on monocytes, macrophages, dendritic cells, and neurons, but not on T or B lymphocytes. Its extracellular ligand is CD47.
CD172a plays roles in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis. This antibody has reported neutralizing activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells
Use ≤ 0.5 µg/test (defined as antibody amount for staining in 100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

SIRPα (CD172a) is a receptor-type transmembrane glycoprotein expressed on myeloid-origin cells such as granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells. It acts as a substrate for activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase-coupled signaling pathways.
Binding to CD47 leads to phosphorylation of ITIM motifs and activation of SHP-1 and SHP-2 phosphatases. This interaction regulates processes such as inhibition of macrophage phagocytosis and T cell/DC activation.
SIRPα influences cellular responses to serum, growth factors, insulin, oncogenes, and adhesion, playing roles in physiological and pathological processes. Cancer cells highly express CD47, activating SIRPα and inhibiting macrophage-mediated tumor cell destruction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지