Thermo Fisher Scientific CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

-

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Flow Cytometry (Flow)

0.06 µg/test

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Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4RA10

Immunogen

Mouse PSGL-1 human IgG1 fusion protein

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Alexa Fluor 647
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848422

Product Specific Information

Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.

Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.