Thermo Fisher Scientific C. botulinum BoNT-B Light Chain Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant Light Chains of BoNT-A, -E, or recombinant Heavy Chains of BoNT-C, or -D is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single-chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins.
The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans.
As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction.
E. coli-expressed recombinant light chains are active proteases but are not toxic because they cannot enter host cells in the absence of the heavy chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.