
Thermo Fisher Scientific Fluorescein Polyclonal Antibody, DyLight 680
DyLight 680으로 결합된 Fluorescein 폴리클로날 항체로, Western blot, ICC/IF, ELISA에 적합. 염소 유래 IgG 기반으로 높은 특이성과 감도를 제공. FITC 검출 및 형광 이미징에 최적화된 연구용 항체.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit visible yellow-green light. This emission ceases upon removal of the excitation light.
Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue, or other biological structure.
FITC is a commonly used marker for antibodies in immunofluorescent techniques since conjugation to proteins is relatively easy and does not destroy biological activity.
Anti-FITC antibodies can be used for isolating FITC-labeled cells by magnetic microbead sorting and for removal of background signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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