Thermo Fisher Scientific RAGE Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1-2 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAGE,uniProtId:Q63495-1,ncbiNodeId:10114,antigenRange:362-380,antigenLength:402,antigenImageFileName:PA1-075_RAGE_Q63495-1_Rabbit.svg,antigenImageFileNamePDP:PA1-075_RAGE_Q63495-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2269243

Product Specific Information

PA1-075 detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples.

PA1-075 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.

The PA1-075 immunogen is a synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. This sequence is 100% conserved in mouse and 70% conserved in humans. This peptide (Cat. # PEP-194) is available for use in neutralization and control experiments.

Target Information

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.