Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), eFluor 450, eBioscience

Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor homologous to T-bet, essential during trophoblast development and gastrulation. In the immune system, Eomes regulates differentiation of effector and memory CD8+ T cells and NK cells, correlating with high CD122 expression.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00). Recommended ≤0.5 µg per test (100 µL final volume). Cell number range: 10^5–10^8 cells/test. Titrate for optimal performance.

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®. Emits at 445 nm and is excited with Violet laser (405 nm). Ensure instrument compatibility.

Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TBR2 belongs to the T-box transcription factor family, involved in developmental regulation. It plays a role in brain development, essential for specification and proliferation of intermediate progenitor cells in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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