
Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), eFluor 450, eBioscience
EOMES 단백질을 인식하는 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. Flow cytometry 및 intracellular staining에 적합하며, 마우스 시료에서 검증되었습니다. Violet laser(405 nm)로 여기되어 445 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 13 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 22 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Dan11mag |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574061 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- eFluor 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor homologous to T-bet, essential during trophoblast development and gastrulation. In the immune system, Eomes regulates differentiation of effector and memory CD8+ T cells and NK cells, correlating with high CD122 expression.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00). Recommended ≤0.5 µg per test (100 µL final volume). Cell number range: 10^5–10^8 cells/test. Titrate for optimal performance.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®. Emits at 445 nm and is excited with Violet laser (405 nm). Ensure instrument compatibility.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TBR2 belongs to the T-box transcription factor family, involved in developmental regulation. It plays a role in brain development, essential for specification and proliferation of intermediate progenitor cells in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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