Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 450, eBioscience

Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• eFluor 660
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Tyrosine phosphorylation is a key mechanism in intracellular signaling cascades. Detection of p-Tyr residues aids in the characterization and purification of phosphorylated proteins and their associated biochemical pathways.

Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by intracellular staining of normal human peripheral blood cells at 5 µL (0.06 µg) per test. A test is defined as the amount of antibody staining a cell sample in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test.

Fluorophore Information:
eFluor™ 450 is an alternative to Pacific Blue™, excited at 405 nm and emitting at 445 nm. Compatible with Violet laser detectors.

Staining Protocols:

• Protocol A: Two-step for cytoplasmic proteins (surface + intracellular detection)
• Protocol B: One-step for nuclear proteins (transcription factors + phosphorylated intracellular proteins)
• Protocol C: Two-step fixation/methanol for phospho-specific signaling discrimination

Detailed protocols available in BestProtocols® - Staining Intracellular Antigens for Flow Cytometry.

Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Tyrosine phosphorylation plays a critical role in mitogenic signal transduction and oncogenic transformation. Antibodies recognizing phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and their signaling pathways. The fluorescein (FITC) conjugate of clone pY20 is particularly useful for direct detection in immunohistochemical and immunocytochemical assays where secondary antibody use may interfere.

For Research Use Only.
Not for diagnostic procedures or resale without authorization.

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