Thermo Fisher Scientific eIF4B Polyclonal Antibody
Thermo Fisher Scientific eIF4B Polyclonal Antibody는 인간 및 마우스 반응성을 가진 Rabbit IgG 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 안정적인 액상 형태로 제공됩니다.
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Thermo Fisher Scientific eIF4B Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–198 of Human EIF4B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547016 |
Product Specific Information
- Recommended positive controls: NT2D1, IMR32, MCF-7, U87-MG, SK-N-SH, SK-N-AS, 293T, A431, HeLa, HepG2, human EIF4B-transfected 293T
- Predicted reactivity: Mouse (97%), Rat (97%), Rhesus Monkey (100%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A, binding near the 5′-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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