Thermo Fisher Scientific ERO1L Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: HCT116, MCF-7, mouse stomach
• Predicted reactivity: Rat (90%), Pig (96%), Rhesus Monkey (99%), Bovine (96%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in the ER membrane as a peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for its retention in the ER and is unique in coupling oxygen reduction to de novo disulfide formation.

The ERO1L enzyme is broadly distributed at low levels in several tissues, with highest levels in the upper digestive tract, and is stimulated by hypoxia via HIF signaling. It generally exists as a monomer but can function as a monomer or homodimer and interacts with PDILT.

After translation, ERO1L becomes N-glycosylated, and the disulfide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accepts an electron from P4HB and funnels it to the active site disulfide Cys-394/Cys-397.

ERO1L acts by oxidizing P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44. It is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis via IP3R1 activation.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.