Thermo Fisher Scientific CD2 Monoclonal Antibody (RPA-2.10), eBioscience
인간 CD2 단백질을 인식하는 RPA-2.10 클론의 단클론 항체로, T세포 및 NK세포 분석에 적합합니다. Flow cytometry, WB, IHC(F) 등 다양한 응용에 사용 가능하며, 비인간 영장류 및 돼지에도 교차 반응합니다. 액상 형태로 4°C 보관하며 연구용으로만 사용됩니다.
- 카탈로그번호
- 14-0029-82
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 5 publications |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay (Functional) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Pig, Rhesus monkey |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-2.10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467044 |
Additional Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
- NovaFluor Yellow 570
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
Description:
The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by most thymocytes, all mature T cells, and a subset of NK cells. CD2 serves as a ligand for CD58 and plays a key role in T-cell adhesion and activation. RPA-2.10 blocks mixed lymphocyte reactions and cross-reacts with non-human primates and pigs.
Applications Reported:
Reported for use in flow cytometry, Western blotting (WB), and immunohistochemical staining of frozen tissue sections. Also used for in vitro blocking of human CD2 (LFA-2). For functional assays, use the Functional Grade purified RPA-2.10 (Product #16-0029-81).
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.5 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD2 (LFA-2) is a monomeric surface antigen (45–58 kDa) expressed on all peripheral blood T cells and some NK cells, but not on B cells. It is among the earliest T-cell markers, present on over 95% of thymocytes. CD2 interacts with CD58 to mediate T-cell adhesion and activation. The CD2–CD58 interaction stabilizes cell-cell contact and enhances immune responses. CD2 deficiency is associated with reduced intestinal inflammation and certain immune disorders.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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