Thermo Fisher Scientific CD1a Monoclonal Antibody (HI149), eBioscience
인간 CD1a 단백질을 인식하는 HI149 단클론 항체로, Flow Cytometry 및 IHC 분석에 적합합니다. 비접합형 포맷이며 높은 순도(>90%)로 정제되었습니다. 인간 시료에 특이적으로 반응하며 항원 제시 연구에 유용합니다.
- 카탈로그번호
- 14-0019-xx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD1a Monoclonal Antibody (HI149), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864806 |
Additional Formats
- eFluor 450 (Cat. 48-0019-42)
- FITC (Cat. 11-0019-42)
- PE (Cat. 12-0019-42)
- PE-Cyanine7 (Cat. 25-0019-42)
- PE-eFluor 610 (Cat. 61-0019-42)
- PerCP-eFluor 710 (Cat. 46-0019-42)
- Custom conjugation available upon request
Product Specific Information
Description:
The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. CD1 family proteins share structural and functional similarities with MHC class I molecules but are non-polymorphic. CD1a associates with β2-microglobulin and plays a role in antigen presentation.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining
Applications Tested:
Tested by flow cytometric analysis using human MOLT-4 cell line. Recommended ≤1 µg per test (100 µL final volume). Optimal titration is advised for specific assay conditions.
Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD1a is a non-polymorphic MHC Class I–related cell surface glycoprotein expressed in association with β2-microglobulin. It is found on cortical thymocytes, Langerhans cells, dendritic cells, and certain T-cell leukemias. CD1a mediates the presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes (CD1a–e), which differ in localization and ligand specificity. CD1a localizes to the plasma membrane and early endocytic vesicles. Alternative splicing variants have been observed but their biological roles remain unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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