Thermo Fisher Scientific CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• APC (Cat. 17-0030-82)
• Biotin (Cat. 13-0030-82)
• FITC (Cat. 11-0030-82)
• Functional Grade (Cat. 16-0030-85)
• PE (Cat. 12-0030-82)
• PerCP-eFluor 710 (Cat. 46-0030-82)
• Custom conjugation available upon request

Product Specific Information

Description:
The eBioG4.18 monoclonal antibody recognizes rat CD3 protein, a key component of the T-cell receptor (TCR) complex and a marker of the T-cell lineage. CD3 mediates signal transduction from the TCR to the nucleus, leading to cellular responses such as IL-2 transcription and clonal expansion.

In vitro, immobilized G4.18 antibody activates T cells, while soluble G4.18 inhibits allogeneic mixed-lymphocyte proliferation and cytotoxicity. In vivo, it can induce long-term tolerance to organ allografts.

Applications Reported:
Reported for use in flow cytometry, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number should be optimized (10⁵–10⁸ cells/test). Antibody titration is advised for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded by closely linked genes on human chromosome 11.

CD3 plays a role in TCR-induced growth arrest, cell survival, and proliferation. It is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells. Decreased T lymphocytes may be observed in certain autoimmune diseases.

Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.